The Basics:

• Start with original, high-quality content.  The “garbage in, garbage out” adage still applies.
• The most widely used desktop player is Adobe Flash Video Player with 98% of computers using it. The player uses .FLV videos ; click here to download Adobe Flash Player 10.
• Alternatively MPEG-4 is a versatile, digital multimedia standard compatible with multiple delivery networks and mobile devices; ideal for use with YouTube, iphone, Blackberry Media Player.
• Resolution: 640 x 480 for SD and 1280 x 720 for HD – Content Delivery Networks (such as YouTube) will resize your video. Upload the original frame size to preserve quality.
• Maintain your original frame rate: SD 29.97 fps,  Click here for HD Frame Rates
• Make sure to maintain the Aspect Ratio – 4:3 or 16:9

Additional Resources:
Online Video: Codecs, Encoding and Compression
SEO Your Videos – Best Practices for Video Search Optimization
Optimal frame dimensions for flash video

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